Journal: EBioMedicine

Article Title: A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests

doi: 10.1016/j.ebiom.2021.103348

Figure Lengend Snippet: Characterisation of cross-reactive IgG responses between SARS-CoV-2 and endemic hCoV strains in the specificity cohorts. (a) Seroreactivity of serum samples from the two specificity cohorts (AIT pre-COVID-19 cohort, n = 210 and MedUni Wien Biobank pre-COVID-19, n = 14) employed for pre-validation of the SARS-CoV-2 tRBD and NP antigens with the Luminex or ELISA assays respectively, was measured with the spike proteins of common-cold hCoVs HKU-1, OC43, 229E and NL63. Outliers were classified as observations that fall above the 75th percentile + 1.5 x IQR. Shades give the respective calculated cut-offs and are color-coded for NP (blue) or tRBD (pink). Values below the box-plots give the measured seropositivity in percent. ( b) Relative IgG levels of NP ( n = 17, blue boxes) and tRBD ( n = 4, pink boxes) outliers towards the spike proteins of hCoV. White boxes give relative IgG levels of sera with readouts <25th percentile ( n = 16 for NP, n = 5 for tRBD) to compare with outliers. Means within groups were compared by One-Way ANOVA followed by a Sidak test to correct for multiple comparisons. c) tRBD and NP-specific seroreactivity of the specificity cohort ( n = 1126 MedUni Wien Biobank) used for clinical validation. Red crosses display sera from individuals with PCR-confirmed hCoV infection. Dashed lines indicate the cut-off of 5 U/mL.

Article Snippet: A recombinant chimeric human/mouse anti-SARS-CoV-2 NP antibody consisting of a mouse scFv fused to the Fc region of human IgG1 (clone 1A6) was purchased from Abcam, Cambridge, UK (#ab272852).

Techniques: Biomarker Discovery, Luminex, Enzyme-linked Immunosorbent Assay, Infection

Journal: Diagnostic Pathology

Article Title: Extramammary Paget disease of the vulva: immunohistochemical analysis of neoangiogenesis and epithelial-mesenchymal transition markers expression

doi: 10.1186/s13000-017-0680-x

Figure Lengend Snippet: a A case with a low value of HVD (microvessels are highlighted by CD31 immunostaining) (original magnification ×200); b A case with a high value of HVD (microvessels are highlighted by CD31 immunostaining) (original magnification ×200); c Same case as B (original magnification ×400); d Paget cells show positive nuclear staining for SLUG; e Positive immunostaining for N-cadherin in Paget cells (original magnification ×200); f Negative immunostaining for N-cadherin in Paget cells (original magnification ×200)

Article Snippet: The following primary antibodies were used: CD34 (monoclonal antibody, clone QBEND/10; 1:400 dilution; Neomarkers, Freemont, CA, USA), CD31 (monoclonal antibody, clone JC70, Prediluted, Cell Marque, Rocklin, CA, US), E-cadherin (monoclonal antibody, clone 36, pre-diluted, Ventana, Tucson, AZ, US), N –cadherin (monoclonal antibody, clone 6G11, dilution 1:50, Dako, Glostrup, Denmark); SLUG (monoclonal antibody, 1A6, dilution 1:100, Novus Biologicals, Littleton, CO, USA), β-catenin (monoclonal antibody, clone 14, ready to use, Cell Marque).

Techniques: Immunostaining, Staining

Journal: Journal of Inflammation Research

Article Title: Interleukin-1 Receptor-Associated Kinase-3 Aggravates Neuroinflammatory Injury After Intracerebral Hemorrhage via Activation NF-κB/IL-17A Pathway in Mice

doi: 10.2147/JIR.S494611

Figure Lengend Snippet: Table of Antibodies Used

Article Snippet: IRAK3 , Rabbit monoclonal , Novus , WB, IF, IHC , WB = 1:2000 IF = 1:200 IHC = 1:50 , NBP1-76782.

Techniques:

Journal: Gut and Liver

Article Title: High Expression of Aldo-Keto Reductase 1B10 Is an Independent Predictor of Favorable Prognosis in Patients with Hepatocellular Carcinoma

doi: 10.5009/gnl13406

Figure Lengend Snippet: Immunostaining of Aldo-Keto Reductase 1B10 showing cytoplasmic immunoreactivity in hepatocellular carcinoma tissue (horse-radish peroxidase stain, ×200).

Article Snippet: The sections were incubated with a primary antibody against AKR1B10 (mouse monoclonal antibody, clone 1A6, 1:800; Abnova Corp., Taipei, Taiwan) for 30 minutes at room temperature.

Techniques: Immunostaining, Staining

Journal: Gut and Liver

Article Title: High Expression of Aldo-Keto Reductase 1B10 Is an Independent Predictor of Favorable Prognosis in Patients with Hepatocellular Carcinoma

doi: 10.5009/gnl13406

Figure Lengend Snippet: Correlation between Aldo-Keto Reductase 1B10 Expression and the Clinicopathologic Features of 255 Hepatocellular Carcinomas

Article Snippet: The sections were incubated with a primary antibody against AKR1B10 (mouse monoclonal antibody, clone 1A6, 1:800; Abnova Corp., Taipei, Taiwan) for 30 minutes at room temperature.

Techniques: Expressing

Journal: Gut and Liver

Article Title: High Expression of Aldo-Keto Reductase 1B10 Is an Independent Predictor of Favorable Prognosis in Patients with Hepatocellular Carcinoma

doi: 10.5009/gnl13406

Figure Lengend Snippet: Univariate and Multivariate Logistic Regression Models for the Prediction of Early Tumor Recurrence in 255 Patients with Hepatocellular Carcinoma

Article Snippet: The sections were incubated with a primary antibody against AKR1B10 (mouse monoclonal antibody, clone 1A6, 1:800; Abnova Corp., Taipei, Taiwan) for 30 minutes at room temperature.

Techniques: Expressing

Journal: Gut and Liver

Article Title: High Expression of Aldo-Keto Reductase 1B10 Is an Independent Predictor of Favorable Prognosis in Patients with Hepatocellular Carcinoma

doi: 10.5009/gnl13406

Figure Lengend Snippet: Univariate Analyses of the Recurrence-Free Survival and the Disease-Specific Survival in 255 Patients with Hepatocellular Carcinoma

Article Snippet: The sections were incubated with a primary antibody against AKR1B10 (mouse monoclonal antibody, clone 1A6, 1:800; Abnova Corp., Taipei, Taiwan) for 30 minutes at room temperature.

Techniques: Expressing

Journal: Gut and Liver

Article Title: High Expression of Aldo-Keto Reductase 1B10 Is an Independent Predictor of Favorable Prognosis in Patients with Hepatocellular Carcinoma

doi: 10.5009/gnl13406

Figure Lengend Snippet: Kaplan-Meier survival curves showing recurrence-free survival (A) and disease-specific survival (B) according to Aldo-Keto Reductase 1B10 expression in 255 hepatocellular carcinomas.

Article Snippet: The sections were incubated with a primary antibody against AKR1B10 (mouse monoclonal antibody, clone 1A6, 1:800; Abnova Corp., Taipei, Taiwan) for 30 minutes at room temperature.

Techniques: Expressing

Journal: Gut and Liver

Article Title: High Expression of Aldo-Keto Reductase 1B10 Is an Independent Predictor of Favorable Prognosis in Patients with Hepatocellular Carcinoma

doi: 10.5009/gnl13406

Figure Lengend Snippet: Multivariate Analyses of the Recurrence-Free Survival and the Disease-Specific Survival in 255 Patients with Hepatocellular Carcinoma

Article Snippet: The sections were incubated with a primary antibody against AKR1B10 (mouse monoclonal antibody, clone 1A6, 1:800; Abnova Corp., Taipei, Taiwan) for 30 minutes at room temperature.

Techniques: Expressing